› 3ori

X-ray diffraction
2Å resolution

Mycobacterium tuberculosis PknB kinase domain L33D mutant (crystal form 1)

Released:
DOI: 10.2210/pdb3ori/pdb
PDB entry 3ori coloured by chain, front view.
PDB entry 3ori coloured by chain, side view.
PDB entry 3ori coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Ra
Primary publication:
Allosteric activation mechanism of the Mycobacterium tuberculosis receptor Ser/Thr protein kinase, PknB.
Lombana TN, Echols N, Good MC, Thomsen ND, Ng HL, Greenstein AE, Falick AM, King DS, Alber T
Structure 18 1667-77 (2010)
PMID: 21134645

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PknB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 311 amino acids
Theoretical weight: 33.52 KDa
Source organism: Mycobacterium tuberculosis H37Ra
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WI81 (Residues: 1-308; Coverage: 49%)
Gene names: MTCY10H4.14c, Rv0014c, pknB
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand AGS 4 x AGS
Ligand MN 8 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 80.931Å b: 51.544Å c: 141.409Å
α: 90° β: 106.62° γ: 90°
R-values:
R R work R free
0.204 0.201 0.258
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Role of eukaryotic-like serine/threonine kinases in bacterial cell division and morphogenesis.
Manuse et al. (2016)
6 more

4 mentions without citation

Mycobacterium tuberculosis Serine/Threonine Protein Kinases.
Prisic et al. (2014)
3 more