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X-ray diffraction
2Å resolution

Crystal structure of Flap endonuclease 1 from hyperthermophilic archaeon Desulfurococcus amylolyticus

Released:
DOI: 10.2210/pdb3ory/pdb
PDB entry 3ory coloured by chain, front view.
PDB entry 3ory coloured by chain, side view.
PDB entry 3ory coloured by chain, top view.
Source organism: Desulfurococcus amylolyticus
Primary publication:
Structure of flap endonuclease 1 from the hyperthermophilic archaeon Desulfurococcus amylolyticus.
Mase T, Kubota K, Miyazono K, Kawarabayasi Y, Tanokura M
Acta Crystallogr Sect F Struct Biol Cryst Commun 67 209-13 (2011)
PMID: 21301087

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-123444 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flap endonuclease 1 Chain: A
Molecule details ›
Chain: A
Length: 363 amino acids
Theoretical weight: 40.88 KDa
Source organism: Desulfurococcus amylolyticus
Expression system: Escherichia coli
UniProt:
  • Canonical: F2Z289 (Residues: 1-363; Coverage: 100%)
Gene name: fen
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P321
Unit cell:
a: 103.76Å b: 103.76Å c: 84.58Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.21 0.225
Expression system: Escherichia coli

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Citations

1 review citation

Haloferax volcanii-a model archaeon for studying DNA replication and repair.
Pérez-Arnaiz et al. (2020)
2 other articles

1 mention without citation

Structure of flap endonuclease 1 from the hyperthermophilic archaeon Desulfurococcus amylolyticus.
Mase et al. (2011)