› 3osp

X-ray diffraction
2.5Å resolution

Structure of rev1

Released:
DOI: 10.2210/pdb3osp/pdb
PDB entry 3osp coloured by chain, front view.
PDB entry 3osp coloured by chain, side view.
PDB entry 3osp coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
DNA synthesis across an abasic lesion by yeast REV1 DNA polymerase.
Nair DT, Johnson RE, Prakash L, Prakash S, Aggarwal AK
J Mol Biol 406 18-28 (2011)
PMID: 21167175

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116673 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA repair protein REV1 Chain: A
Molecule details ›
Chain: A
Length: 434 amino acids
Theoretical weight: 49.45 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P12689 (Residues: 305-738; Coverage: 44%)
Gene names: O6339, REV1, YOR346W
Sequence domains:
Structure domains:
5'-D(*TP*AP*AP*(3DR)P*GP*TP*AP*GP*GP*GP*GP*AP*GP*GP*AP*T)-3' Chain: T
Molecule details ›
Chain: T
Length: 16 nucleotides
Theoretical weight: 4.92 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
5'-D(*AP*TP*CP*CP*TP*CP*CP*CP*CP*TP*AP*(DOC))-3' Chain: P
Molecule details ›
Chain: P
Length: 12 nucleotides
Theoretical weight: 3.5 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand DCP 1 x DCP
Ligand MG 2 x MG
1 modified residue:
Ligand DOC 1 x DOC

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2221
Unit cell:
a: 180.021Å b: 200.236Å c: 55.725Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.204 0.255
Expression systems:
  • Saccharomyces cerevisiae
  • Not provided

Quick links

Citations

7 review citations

DNA repair mechanisms and the bypass of DNA damage in Saccharomyces cerevisiae.
Boiteux et al. (2013)
6 more

4 mentions without citation

Eukaryotic translesion synthesis: Choosing the right tool for the job.
Powers et al. (2018)
3 more