› 3ot2

X-ray diffraction
1.96Å resolution

Crystal structure of a putative nuclease belonging to DUF820 (Ava_3926) from Anabaena variabilis ATCC 29413 at 1.96 A resolution

Released:
DOI: 10.2210/pdb3ot2/pdb
PDB entry 3ot2 coloured by chain, front view.
PDB entry 3ot2 coloured by chain, side view.
PDB entry 3ot2 coloured by chain, top view.
Source organism: Trichormus variabilis ATCC 29413
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174749 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative restriction endonuclease domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 187 amino acids
Theoretical weight: 21.34 KDa
Source organism: Trichormus variabilis ATCC 29413
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3M655 (Residues: 1-186; Coverage: 100%)
Gene name: Ava_3926
Sequence domains: Putative restriction endonuclease
Structure domains: tt1808, chain A

Ligands and Environments

4 bound ligands:
Ligand ACT 2 x ACT
Ligand BU1 1 x BU1
Ligand CL 2 x CL
Ligand EDO 6 x EDO
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P23
Unit cell:
a: 119.6Å b: 119.6Å c: 119.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.216 0.239
Expression system: Escherichia coli

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