› 3ou6

X-ray diffraction
2.3Å resolution

DhpI-SAM complex

Released:
DOI: 10.2210/pdb3ou6/pdb
PDB entry 3ou6 coloured by chain, front view.
PDB entry 3ou6 coloured by chain, side view.
PDB entry 3ou6 coloured by chain, top view.
Source organism: Streptomyces luridus
Primary publication:
Characterization and structure of DhpI, a phosphonate O-methyltransferase involved in dehydrophos biosynthesis.
Lee JH, Bae B, Kuemin M, Circello BT, Metcalf WW, Nair SK, van der Donk WA
Proc Natl Acad Sci U S A 107 17557-62 (2010)
PMID: 20876132

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112861 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methyltransferase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 218 amino acids
Theoretical weight: 24.61 KDa
Source organism: Streptomyces luridus
Expression system: Escherichia coli
UniProt:
  • Canonical: D7PC21 (Residues: 1-218; Coverage: 100%)
Gene name: dhpI
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

2 bound ligands:
Ligand SAM 4 x SAM
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P6122
Unit cell:
a: 166.428Å b: 166.428Å c: 168.076Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.19 0.237
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Architectures, mechanisms and molecular evolution of natural product methyltransferases.
Liscombe et al. (2012)
1 more

3 mentions without citation

Characterization and structure of DhpI, a phosphonate O-methyltransferase involved in dehydrophos biosynthesis.
Lee et al. (2010)
2 more