› 3ouf

X-ray diffraction
1.55Å resolution

Structure of a K+ selective NaK mutant

Released:
DOI: 10.2210/pdb3ouf/pdb
PDB entry 3ouf coloured by chain, front view.
PDB entry 3ouf coloured by chain, side view.
PDB entry 3ouf coloured by chain, top view.
Source organism: Bacillus cereus m1550
Primary publication:
Tuning the ion selectivity of tetrameric cation channels by changing the number of ion binding sites.
Derebe MG, Sauer DB, Zeng W, Alam A, Shi N, Jiang Y
Proc Natl Acad Sci U S A 108 598-602 (2011)
PMID: 21187421

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-228126 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Potassium channel protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 97 amino acids
Theoretical weight: 10.77 KDa
Source organism: Bacillus cereus m1550
Expression system: Escherichia coli
Structure domains: Helix Hairpins

Ligands and Environments

2 bound ligands:
Ligand MPD 5 x MPD
Ligand K 9 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I4
Unit cell:
a: 67.939Å b: 67.939Å c: 89.638Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.215
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Bacterial voltage-gated sodium channels (BacNa(V)s) from the soil, sea, and salt lakes enlighten molecular mechanisms of electrical signaling and pharmacology in the brain and heart.
Payandeh et al. (2015)
12 more

15 mentions without citation

Tuning the ion selectivity of tetrameric cation channels by changing the number of ion binding sites.
Derebe et al. (2011)
14 more