› 3oxp

X-ray diffraction
1.2Å resolution

Structure of phosphotransferase enzyme II, A component from Yersinia pestis CO92 at 1.2 A resolution

Released:
DOI: 10.2210/pdb3oxp/pdb
PDB entry 3oxp coloured by chain, front view.
PDB entry 3oxp coloured by chain, side view.
PDB entry 3oxp coloured by chain, top view.
Source organism: Yersinia pestis CO92
Entry authors: Filippova EV, Wawrzak Z, Kudritska M, Edwards A, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236770 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphotransferase enzyme II, A component Chains: A, B
Molecule details ›
Chains: A, B
Length: 150 amino acids
Theoretical weight: 16.22 KDa
Source organism: Yersinia pestis CO92
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2U2GUN8 (Residues: 1-147; Coverage: 100%)
Gene name: YPO2569
Sequence domains: Phosphoenolpyruvate-dependent sugar phosphotransferase system, EIIA 2
Structure domains: Mannitol-specific EII; Chain A

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 56.906Å b: 57.946Å c: 78.742Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.147 0.179
Expression system: Escherichia coli

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