› 3ozu

X-ray diffraction
2Å resolution

The Crystal Structure of flavohemoglobin from R. eutrophus in complex with miconazole

Released:
DOI: 10.2210/pdb3ozu/pdb
PDB entry 3ozu coloured by chain, front view.
PDB entry 3ozu coloured by chain, side view.
PDB entry 3ozu coloured by chain, top view.
Source organism: Cupriavidus necator H16
Primary publication:
Structure of Ralstonia eutropha flavohemoglobin in complex with three antibiotic azole compounds.
El Hammi E, Warkentin E, Demmer U, Limam F, Marzouki NM, Ermler U, Baciou L
Biochemistry 50 1255-64 (2011)
PMID: 21210640

Function and Biology Details

Reaction catalysed: 
2 nitric oxide + 2 O(2) + NAD(P)H = 2 nitrate + NAD(P)(+) + H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-153921 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavohemoprotein Chain: A
Molecule details ›
Chain: A
Length: 403 amino acids
Theoretical weight: 44.83 KDa
Source organism: Cupriavidus necator H16
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P39662 (Residues: 1-403; Coverage: 100%)
Gene names: PHG200, fhp, hmp
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD

Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand X89 1 x X89
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 121.339Å b: 105.278Å c: 46.445Å
α: 90° β: 101.9° γ: 90°
R-values:
R R work R free
0.188 0.185 0.233
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Protection from nitrosative stress: a central role for microbial flavohemoglobin.
Forrester et al. (2012)
2 more

2 mentions without citation

Synthesis, characterization, in silico molecular docking, and antibacterial activities of some new nitrogen-heterocyclic analogues based on a p-phenolic unit.
Hussein et al. (2022)
1 more