› 3p03

X-ray diffraction
3.35Å resolution

Crystal structure of BetP-G153D with choline bound

Released:
DOI: 10.2210/pdb3p03/pdb
PDB entry 3p03 coloured by chain, front view.
PDB entry 3p03 coloured by chain, side view.
PDB entry 3p03 coloured by chain, top view.
Source organism: Corynebacterium glutamicum
Primary publication:
Substrate specificity and ion coupling in the Na+/betaine symporter BetP.
Perez C, Koshy C, Ressl S, Nicklisch S, Krämer R, Ziegler C
EMBO J 30 1221-9 (2011)
PMID: 21364531

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157051 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine betaine transporter BetP Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 566 amino acids
Theoretical weight: 61.06 KDa
Source organism: Corynebacterium glutamicum
Expression system: Escherichia coli
UniProt:
  • Canonical: P54582 (Residues: 30-595; Coverage: 95%)
Gene names: Cgl0892, betP, cg1016
Sequence domains: BCCT, betaine/carnitine/choline family transporter

Ligands and Environments

1 bound ligand:
Ligand CHT 1 x CHT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 117.56Å b: 129.31Å c: 183.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.245 0.3
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Regulation of Amino Acid Transport in Saccharomyces cerevisiae.
Bianchi et al. (2019)
4 more

7 mentions without citation

Transporter oligomerization: form and function.
Alguel et al. (2016)
6 more