› 3p09

X-ray diffraction
1.9Å resolution

Crystal Structure of Beta-Lactamase from Francisella tularensis

Released:
DOI: 10.2210/pdb3p09/pdb
PDB entry 3p09 coloured by chain, front view.
PDB entry 3p09 coloured by chain, side view.
PDB entry 3p09 coloured by chain, top view.
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Entry authors: Kim Y, Makowska-Grzyska M, Hasseman J, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-177699 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 290 amino acids
Theoretical weight: 32.43 KDa
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5NH60 (Residues: 1-287; Coverage: 100%)
Gene name: FTT_0611c
Sequence domains: Beta-lactamase enzyme family
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand SO4 7 x SO4
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 77.652Å b: 143.466Å c: 46.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.207
Expression system: Escherichia coli

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