› 3p2f

X-ray diffraction
2.3Å resolution

Crystal structure of TofI in an apo form

Released:
DOI: 10.2210/pdb3p2f/pdb
PDB entry 3p2f coloured by chain, front view.
PDB entry 3p2f coloured by chain, side view.
PDB entry 3p2f coloured by chain, top view.
Source organism: Burkholderia glumae
Primary publication:
Small-molecule inhibitor binding to an N-acyl-homoserine lactone synthase.
Chung J, Goo E, Yu S, Choi O, Lee J, Kim J, Kim H, Igarashi J, Suga H, Moon JS, Hwang I, Rhee S
Proc Natl Acad Sci U S A 108 12089-94 (2011)
PMID: 21730159

Function and Biology Details

Reaction catalysed: 
An acyl-[acyl-carrier-protein] + S-adenosyl-L-methionine = [acyl-carrier-protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175780 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acyl-homoserine-lactone synthase Chain: A
Molecule details ›
Chain: A
Length: 201 amino acids
Theoretical weight: 22.22 KDa
Source organism: Burkholderia glumae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4VSJ8 (Residues: 1-203; Coverage: 99%)
Gene names: bglu_2g14490, tofI
Sequence domains: Autoinducer synthase
Structure domains: Aminopeptidase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6C1
Spacegroup: P3221
Unit cell:
a: 65.971Å b: 65.971Å c: 104.768Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.228 0.27
Expression system: Escherichia coli

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Citations

22 review citations

Exploiting quorum sensing to confuse bacterial pathogens.
LaSarre et al. (2013)
21 more

6 mentions without citation

Structure-based Druggability Assessment of Anti-virulence Targets from Pseudomonas aeruginosa.
Froes et al. (2019)
5 more