› 3p30

X-ray diffraction
3.3Å resolution

crystal structure of the cluster II Fab 1281 in complex with HIV-1 gp41 ectodomain

Released:
DOI: 10.2210/pdb3p30/pdb
PDB entry 3p30 coloured by chain, front view.
PDB entry 3p30 coloured by chain, side view.
PDB entry 3p30 coloured by chain, top view.
Source organisms:
Primary publication:
Distinct conformational states of HIV-1 gp41 are recognized by neutralizing and non-neutralizing antibodies.
Frey G, Chen J, Rits-Volloch S, Freeman MM, Zolla-Pazner S, Chen B
Nat Struct Mol Biol 17 1486-91 (2010)
PMID: 21076402

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-211638 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HIV-1 gp41 Chain: A
Molecule details ›
Chain: A
Length: 96 amino acids
Theoretical weight: 11.18 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli BL21(DE3)
Structure domains: Helix Hairpins
1281 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 22.62 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
1281 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 226 amino acids
Theoretical weight: 23.98 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: R3
Unit cell:
a: 115.83Å b: 115.83Å c: 119.544Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.262 0.261 0.289
Expression systems:
  • Escherichia coli BL21(DE3)
  • Homo sapiens

Quick links

Citations

4 review citations

Human immunodeficiency virus (HIV) immunopathogenesis and vaccine development: a review.
Girard et al. (2011)
3 more

15 mentions without citation

HIV-1 envelope glycoprotein structure.
Merk et al. (2013)
14 more