› 3p3q

X-ray diffraction
2.4Å resolution

Crystal Structure of MmoQ Response regulator from Methylococcus capsulatus str. Bath at the resolution 2.4A, Northeast Structural Genomics Consortium Target McR175M

Released:
DOI: 10.2210/pdb3p3q/pdb
PDB entry 3p3q coloured by chain, front view.
PDB entry 3p3q coloured by chain, side view.
PDB entry 3p3q coloured by chain, top view.
Source organism: Methylococcus capsulatus
Entry authors: Kuzin AP, Vorobiev SM, Abashidze M, Seetharaman J, Janjua J, Xiao R, Foote EL, Ciccosanti C, Wang H, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-182162 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
diguanylate cyclase Chains: A, B
Molecule details ›
Chains: A, B
Length: 283 amino acids
Theoretical weight: 31.23 KDa
Source organism: Methylococcus capsulatus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7WZ31 (Residues: 29-302; Coverage: 43%)
Gene name: mmoQ
Sequence domains: HDOD domain
Structure domains: Hypothetical protein af1432

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand NA 1 x NA
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 44.231Å b: 76.156Å c: 160.281Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.255
Expression system: Escherichia coli BL21(DE3)

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