› 3p3v

X-ray diffraction
1.65Å resolution

Crystal structure of a PTS dependent N-acetyl-galactosamine-IIB component (agaV, SPy_0631) from Streptococcus pyogenes at 1.65 A resolution

Released:
DOI: 10.2210/pdb3p3v/pdb
PDB entry 3p3v coloured by chain, front view.
PDB entry 3p3v coloured by chain, side view.
PDB entry 3p3v coloured by chain, top view.
Source organism: Streptococcus pyogenes serotype M1
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS EIIB type-4 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 163 amino acids
Theoretical weight: 18.09 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9A0T4 (Residues: 1-162; Coverage: 100%)
Gene names: SPy_0631, agaV
Sequence domains: PTS system sorbose subfamily IIB component
Structure domains: Phosphotransferase system, sorbose subfamily IIB component

Ligands and Environments

2 bound ligands:
Ligand PEG 3 x PEG
Ligand PGE 1 x PGE
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 42.139Å b: 80.696Å c: 47.213Å
α: 90° β: 105.26° γ: 90°
R-values:
R R work R free
0.16 0.158 0.194
Expression system: Escherichia coli

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