› 3p42

X-ray diffraction
1.91Å resolution

Structure of GfcC (YmcB), protein encoded by the E. coli group 4 capsule operon

Released:
DOI: 10.2210/pdb3p42/pdb
PDB entry 3p42 coloured by chain, front view.
PDB entry 3p42 coloured by chain, side view.
PDB entry 3p42 coloured by chain, top view.
Source organism: Escherichia coli O127:H6 str. E2348/69
Primary publication:
The crystal structure of Escherichia coli group 4 capsule protein GfcC reveals a domain organization resembling that of Wza.
Sathiyamoorthy K, Mills E, Franzmann TM, Rosenshine I, Saper MA
Biochemistry 50 5465-76 (2011)
PMID: 21449614

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-110629 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Predicted protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 236 amino acids
Theoretical weight: 26.27 KDa
Source organism: Escherichia coli O127:H6 str. E2348/69
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B7UN63 (Residues: 22-248; Coverage: 92%)
Gene names: E2348C_0970, gfcC
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 68.831Å b: 99.98Å c: 69.02Å
α: 90° β: 91.74° γ: 90°
R-values:
R R work R free
0.175 0.173 0.217
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Experimental evidence validating the computational inference of functional associations from gene fusion events: a critical survey.
Promponas et al. (2014)
15 other articles

3 mentions without citation

An integrated linkage, chromosome, and genome map for the yellow fever mosquito Aedes aegypti.
Timoshevskiy et al. (2013)
2 more