› 3p4c

X-ray diffraction
1.15Å resolution

Alternatingly modified 2'Fluoro RNA octamer f/rA2U2-R32

Released:
DOI: 10.2210/pdb3p4c/pdb
PDB entry 3p4c coloured by chain, front view.
PDB entry 3p4c coloured by chain, side view.
PDB entry 3p4c coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Unexpected origins of the enhanced pairing affinity of 2'-fluoro-modified RNA.
Pallan PS, Greene EM, Jicman PA, Pandey RK, Manoharan M, Rozners E, Egli M
Nucleic Acids Res 39 3482-95 (2011)
PMID: 21183463

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-196716 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
5'-R(*(CFZ)P*GP*(AF2)P*AP*(UFT)P*UP*(CFZ)P*G)-3' Chains: A, B
Molecule details ›
Chains: A, B
Length: 8 nucleotides
Theoretical weight: 2.53 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SR 3 x SR
3 modified residues:
Ligand CFZ 4 x CFZ
Ligand AF2 2 x AF2
Ligand UFT 2 x UFT

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: R32
Unit cell:
a: 40.55Å b: 40.55Å c: 117.222Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.182 0.249
Expression system: Not provided

Quick links

Citations

23 review citations

Designing chemically modified oligonucleotides for targeted gene silencing.
Deleavey et al. (2012)
22 more

2 mentions without citation

Unexpected origins of the enhanced pairing affinity of 2'-fluoro-modified RNA.
Pallan et al. (2011)
1 more