› 3p56

X-ray diffraction
4.06Å resolution

The structure of the human RNase H2 complex defines key interaction interfaces relevant to enzyme function and human disease

Released:
DOI: 10.2210/pdb3p56/pdb
PDB entry 3p56 coloured by chain, front view.
PDB entry 3p56 coloured by chain, side view.
PDB entry 3p56 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of the human RNase H2 complex defines key interaction interfaces relevant to enzyme function and human disease.
Reijns MA, Bubeck D, Gibson LC, Graham SC, Baillie GS, Jones EY, Jackson AP
J Biol Chem 286 10530-9 (2011)
PMID: 21177854

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131120 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ribonuclease H2 subunit A Chains: A, D
Molecule details ›
Chains: A, D
Length: 299 amino acids
Theoretical weight: 33.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O75792 (Residues: 1-299; Coverage: 100%)
Gene names: RNASEH2A, RNASEHI, RNHIA
Sequence domains: Ribonuclease HII
Ribonuclease H2 subunit B Chains: B, E
Molecule details ›
Chains: B, E
Length: 237 amino acids
Theoretical weight: 26.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5TBB1 (Residues: 2-226; Coverage: 72%)
Gene names: DLEU8, RNASEH2B
Sequence domains:
Ribonuclease H2 subunit C Chains: C, F
Molecule details ›
Chains: C, F
Length: 164 amino acids
Theoretical weight: 17.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8TDP1 (Residues: 1-164; Coverage: 100%)
Gene names: AYP1, RNASEH2C
Sequence domains: Ribonuclease H2 non-catalytic subunit (Ylr154p-like)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 212.238Å b: 42.302Å c: 186.95Å
α: 90° β: 98.11° γ: 90°
R-values:
R R work R free
0.375 0.374 0.379
Expression system: Escherichia coli BL21

Quick links

Citations

9 review citations

Processing ribonucleotides incorporated during eukaryotic DNA replication.
Williams et al. (2016)
8 more

3 mentions without citation

The structure of the human RNase H2 complex defines key interaction interfaces relevant to enzyme function and human disease.
Reijns et al. (2011)
2 more