› 3p5n

X-ray diffraction
3.6Å resolution

Structure and mechanism of the S component of a bacterial ECF transporter

Released:
DOI: 10.2210/pdb3p5n/pdb
PDB entry 3p5n coloured by chain, front view.
PDB entry 3p5n coloured by chain, side view.
PDB entry 3p5n coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus TCH60
Primary publication:
Structure and mechanism of the S component of a bacterial ECF transporter.
Zhang P, Wang J, Shi Y
Nature 468 717-20 (2010)
PMID: 20972419

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Riboflavin transporter RibU Chains: A, B
Molecule details ›
Chains: A, B
Length: 189 amino acids
Theoretical weight: 21.03 KDa
Source organism: Staphylococcus aureus subsp. aureus TCH60
Expression system: Escherichia coli
UniProt:
  • Canonical: E5QVT2 (Residues: 1-189; Coverage: 100%)
Gene names: HMPREF0772_11721, ribU
Sequence domains: ECF transporter, substrate-specific component
Structure domains: Arp2/3 complex 21 kDa subunit ARPC3

Ligands and Environments

1 bound ligand:
Ligand RBF 2 x RBF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 50.434Å b: 94.244Å c: 115.402Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.266 0.265 0.289
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Structural Biology of the Major Facilitator Superfamily Transporters.
Yan N. (2015)
9 more

14 mentions without citation

Structural diversity of ABC transporters.
ter Beek et al. (2014)
13 more