› 3p67

X-ray diffraction
1.5Å resolution

T26S mutant of pentaerythritol tetranitrate reductase containing a bound acetate molecule

Released:
DOI: 10.2210/pdb3p67/pdb
PDB entry 3p67 coloured by chain, front view.
PDB entry 3p67 coloured by chain, side view.
PDB entry 3p67 coloured by chain, top view.
Source organism: Enterobacter cloacae
Primary publication:
Focused directed evolution of pentaerythritol tetranitrate reductase by using automated anaerobic kinetic screening of site-saturated libraries.
Hulley ME, Toogood HS, Fryszkowska A, Mansell D, Stephens GM, Gardiner JM, Scrutton NS
Chembiochem 11 2433-47 (2010)
PMID: 21064170

Function and Biology Details

Reaction catalysed: 
NADPH + acceptor = NADP(+) + reduced acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159784 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 373 amino acids
Theoretical weight: 40.63 KDa
Source organism: Enterobacter cloacae
Expression system: Escherichia coli
UniProt:
  • Canonical: P71278 (Residues: 1-365; Coverage: 100%)
Gene name: onr
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 56.919Å b: 69.063Å c: 88.871Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.146 0.171
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Applications of protein engineering to members of the old yellow enzyme family.
Amato et al. (2015)
1 more