› 3p7j

X-ray diffraction
2.3Å resolution

Drosophila HP1a chromo shadow domain

Released:
DOI: 10.2210/pdb3p7j/pdb
PDB entry 3p7j coloured by chain, front view.
PDB entry 3p7j coloured by chain, side view.
PDB entry 3p7j coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
The HP1a disordered C terminus and chromo shadow domain cooperate to select target peptide partners.
Mendez DL, Kim D, Chruszcz M, Stephens GE, Minor W, Khorasanizadeh S, Elgin SC
Chembiochem 12 1084-96 (2011)
PMID: 21472955

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heterochromatin protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 87 amino acids
Theoretical weight: 10.04 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05205 (Residues: 131-206; Coverage: 37%)
Gene names: CG8409, HP1, HP1a, Su(var)2-5, Su(var)205
Sequence domains: Chromo shadow domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 44.755Å b: 56.545Å c: 67.295Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

HP1a: a structural chromosomal protein regulating transcription.
Eissenberg et al. (2014)
8 more

6 mentions without citation

Insights into HP1a-Chromatin Interactions.
Meyer-Nava et al. (2020)
5 more