› 3p7m

X-ray diffraction
2.2Å resolution

Structure of putative lactate dehydrogenase from Francisella tularensis subsp. tularensis SCHU S4

Released:
DOI: 10.2210/pdb3p7m/pdb
PDB entry 3p7m coloured by chain, front view.
PDB entry 3p7m coloured by chain, side view.
PDB entry 3p7m coloured by chain, top view.
Source organism: Francisella tularensis subsp. tularensis
Entry authors: Osinski T, Cymborowski M, Zimmerman MD, Gordon E, Grimshaw S, Skarina T, Chruszcz M, Savchenko A, Anderson W, Minor W, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = oxaloacetate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184465 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 321 amino acids
Theoretical weight: 34.92 KDa
Source organism: Francisella tularensis subsp. tularensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8G942 (Residues: 1-319; Coverage: 100%)
Gene name: mdh
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
1 modified residue:
Ligand MSE 52 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 78.74Å b: 99.147Å c: 83.345Å
α: 90° β: 103.54° γ: 90°
R-values:
R R work R free
0.185 0.182 0.233
Expression system: Escherichia coli

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