› 3p7n

X-ray diffraction
2.1Å resolution

Crystal structure of light activated transcription factor El222 from Erythrobacter litoralis

Released:
DOI: 10.2210/pdb3p7n/pdb
PDB entry 3p7n coloured by chain, front view.
PDB entry 3p7n coloured by chain, side view.
PDB entry 3p7n coloured by chain, top view.
Source organism: Erythrobacter litoralis HTCC2594
Primary publication:
Structural basis of photosensitivity in a bacterial light-oxygen-voltage/helix-turn-helix (LOV-HTH) DNA-binding protein.
Nash AI, McNulty R, Shillito ME, Swartz TE, Bogomolni RA, Luecke H, Gardner KH
Proc Natl Acad Sci U S A 108 9449-54 (2011)
PMID: 21606338

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173852 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Light-activated DNA-binding protein EL222 Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 28.55 KDa
Source organism: Erythrobacter litoralis HTCC2594
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2NB98 (Residues: 4-225; Coverage: 99%)
Gene name: ELI_04755
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FMN 2 x FMN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 37.044Å b: 81.595Å c: 65.478Å
α: 90° β: 94.57° γ: 90°
R-values:
R R work R free
0.266 0.263 0.328
Expression system: Escherichia coli

Quick links

Citations

31 review citations

Function, structure and mechanism of bacterial photosensory LOV proteins.
Herrou et al. (2011)
30 more

12 mentions without citation

Photochemistry of flavoprotein light sensors.
Conrad et al. (2014)
11 more