› 3paa

X-ray diffraction
1.9Å resolution

Mechanism of inactivation of E. coli aspartate aminotransferase by (S)-4-amino-4,5-dihydro-2-furancarboxylic acid (S-ADFA) pH 8.0

Released:
DOI: 10.2210/pdb3paa/pdb
PDB entry 3paa coloured by chain, front view.
PDB entry 3paa coloured by chain, side view.
PDB entry 3paa coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Mechanism of inactivation of Escherichia coli aspartate aminotransferase by (S)-4-amino-4,5-dihydro-2-furancarboxylic acid .
Liu D, Pozharski E, Fu M, Silverman RB, Ringe D
Biochemistry 49 10507-15 (2010)
PMID: 21033689

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112493 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminotransferase Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 43.62 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: D3H0F7 (Residues: 1-396; Coverage: 100%)
Gene names: EC042_1019, aspC
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand PJ7 1 x PJ7
Ligand PMP 1 x PMP
Ligand GOL 11 x GOL
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2221
Unit cell:
a: 154.846Å b: 85.769Å c: 79.005Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.139 0.137 0.184
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Design and Mechanism of GABA Aminotransferase Inactivators. Treatments for Epilepsies and Addictions.
Silverman RB. (2018)
4 other articles

1 mention without citation

Mechanism of inactivation of Escherichia coli aspartate aminotransferase by (S)-4-amino-4,5-dihydro-2-furancarboxylic acid .
Liu et al. (2010)