› 3pau

X-ray diffraction
2Å resolution

CueO in the resting oxidized state

Released:
DOI: 10.2210/pdb3pau/pdb
PDB entry 3pau coloured by chain, front view.
PDB entry 3pau coloured by chain, side view.
PDB entry 3pau coloured by chain, top view.
Source organism: Escherichia coli str. K-12 substr. W3110
Entry authors: Roberts SA, Singh S, Montfort WR

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multicopper oxidase CueO Chain: A
Molecule details ›
Chain: A
Length: 488 amino acids
Theoretical weight: 53.48 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P36649 (Residues: 29-516; Coverage: 100%)
Gene names: JW0119, b0123, cueO, yacK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand C2O 1 x C2O
Ligand CU 2 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: C2221
Unit cell:
a: 69.216Å b: 73.113Å c: 183.784Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.215 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links