› 3pay

X-ray diffraction
2.5Å resolution

Crystal structure of a putative adhesin (BACOVA_04077) from Bacteroides ovatus at 2.50 A resolution

Released:
DOI: 10.2210/pdb3pay/pdb
PDB entry 3pay coloured by chain, front view.
PDB entry 3pay coloured by chain, side view.
PDB entry 3pay coloured by chain, top view.
Source organism: Bacteroides ovatus ATCC 8483
Primary publication:
A Distinct Type of Pilus from the Human Microbiome.
Xu Q, Shoji M, Shibata S, Naito M, Sato K, Elsliger MA, Grant JC, Axelrod HL, Chiu HJ, Farr CL, Jaroszewski L, Knuth MW, Deacon AM, Godzik A, Lesley SA, Curtis MA, Nakayama K, Wilson IA
Cell 165 690-703 (2016)
PMID: 27062925

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108347 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FimB/Mfa2 family fimbrial subunit Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 314 amino acids
Theoretical weight: 36.08 KDa
Source organism: Bacteroides ovatus ATCC 8483
Expression system: Escherichia coli
UniProt:
  • Canonical: A7M1U3 (Residues: 20-332; Coverage: 100%)
Gene name: BACOVA_04077
Sequence domains: Fimbrillin-A associated anchor proteins Mfa1 and Mfa2
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PEG 3 x PEG
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P1
Unit cell:
a: 67.107Å b: 78.776Å c: 84.105Å
α: 93.88° β: 102.08° γ: 103.54°
R-values:
R R work R free
0.215 0.214 0.239
Expression system: Escherichia coli

Quick links

Citations

10 review citations

The Type IX Secretion System (T9SS): Highlights and Recent Insights into Its Structure and Function.
Lasica et al. (2017)
9 more

1 mention without citation

Structural and functional characterization of shaft, anchor, and tip proteins of the Mfa1 fimbria from the periodontal pathogen Porphyromonas gingivalis.
Hall et al. (2018)