› 3pbr

X-ray diffraction
1.95Å resolution

Crystal structure of PBP3 complexed with meropenem

Released:
DOI: 10.2210/pdb3pbr/pdb
PDB entry 3pbr coloured by chain, front view.
PDB entry 3pbr coloured by chain, side view.
PDB entry 3pbr coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structural basis for effectiveness of siderophore-conjugated monocarbams against clinically relevant strains of Pseudomonas aeruginosa.
Han S, Zaniewski RP, Marr ES, Lacey BM, Tomaras AP, Evdokimov A, Miller JR, Shanmugasundaram V
Proc Natl Acad Sci U S A 107 22002-7 (2010)
PMID: 21135211

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124404 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidoglycan D,D-transpeptidase FtsI Chain: A
Molecule details ›
Chain: A
Length: 538 amino acids
Theoretical weight: 58.33 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: G3XD46 (Residues: 50-579; Coverage: 92%)
Gene names: PA4418, ftsI, pbpB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MER 1 x MER
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 68.248Å b: 80.465Å c: 87.489Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.247
Expression system: Escherichia coli

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Citations

29 review citations

Antibacterial drug discovery in the resistance era.
Brown et al. (2016)
28 more

9 mentions without citation

Mobile Carbapenemase Genes in Pseudomonas aeruginosa.
Yoon et al. (2021)
8 more