› 3pei

X-ray diffraction
2.7Å resolution

Crystal Structure of Cytosol Aminopeptidase from Francisella tularensis

Released:
DOI: 10.2210/pdb3pei/pdb
PDB entry 3pei coloured by chain, front view.
PDB entry 3pei coloured by chain, side view.
PDB entry 3pei coloured by chain, top view.
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Entry authors: Maltseva N, Kim Y, Gu M, Anderson WF, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reactions catalysed: 
Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177661 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable cytosol aminopeptidase Chain: A
Molecule details ›
Chain: A
Length: 486 amino acids
Theoretical weight: 53.95 KDa
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5NFC1 (Residues: 1-479; Coverage: 100%)
Gene names: FTT_1318c, pepA
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand K 3 x K
Ligand EDO 10 x EDO
Ligand PEG 2 x PEG
Ligand CL 5 x CL
Ligand FMT 3 x FMT
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 21 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6322
Unit cell:
a: 161.483Å b: 161.483Å c: 105.27Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.165 0.21
Expression system: Escherichia coli BL21

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