› 3pf5

X-ray diffraction
1.68Å resolution

Crystal structure of Bs-CspB in complex with rU6

Released:
DOI: 10.2210/pdb3pf5/pdb
PDB entry 3pf5 coloured by chain, front view.
PDB entry 3pf5 coloured by chain, side view.
PDB entry 3pf5 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
RNA single strands bind to a conserved surface of the major cold shock protein in crystals and solution.
Sachs R, Max KE, Heinemann U, Balbach J
RNA 18 65-76 (2012)
PMID: 22128343

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152198 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Cold shock protein CspB Chains: A, B
Molecule details ›
Chains: A, B
Length: 67 amino acids
Theoretical weight: 7.37 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32081 (Residues: 1-67; Coverage: 100%)
Gene names: BSU09100, cspA, cspB
Sequence domains: 'Cold-shock' DNA-binding domain
Structure domains: Nucleic acid-binding proteins
hexaribouracil (rU6) Chains: R, S
Molecule details ›
Chains: R, S
Length: 6 nucleotides
Theoretical weight: 1.79 KDa
Source organism: Bacillus subtilis
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 49.038Å b: 49.769Å c: 57.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.235
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

8 review citations

How RNA-Binding Proteins Interact with RNA: Molecules and Mechanisms.
Corley et al. (2020)
7 more

8 mentions without citation

Cold-Shock Domains-Abundance, Structure, Properties, and Nucleic-Acid Binding.
Heinemann et al. (2021)
7 more