› 3pfq

X-ray diffraction
4Å resolution

Crystal Structure and Allosteric Activation of Protein Kinase C beta II

Released:
DOI: 10.2210/pdb3pfq/pdb
PDB entry 3pfq coloured by chain, front view.
PDB entry 3pfq coloured by chain, side view.
PDB entry 3pfq coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystal structure and allosteric activation of protein kinase C βII.
Leonard TA, Różycki B, Saidi LF, Hummer G, Hurley JH
Cell 144 55-66 (2011)
PMID: 21215369

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159394 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein kinase C beta type Chain: A
Molecule details ›
Chain: A
Length: 674 amino acids
Theoretical weight: 77.25 KDa
Source organism: Rattus norvegicus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P68403 (Residues: 1-668; Coverage: 99%)
Gene names: Pkcb, Prkcb, Prkcb1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 3 x CA
Ligand ZN 2 x ZN
Ligand ANP 1 x ANP
2 modified residues:
Ligand TPO 2 x TPO
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P3221
Unit cell:
a: 114.27Å b: 114.27Å c: 170.84Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.244 0.193 0.244
Expression system: Trichoplusia ni

Quick links

Citations

26 review citations

The structural basis for control of eukaryotic protein kinases.
Endicott et al. (2012)
25 more

35 mentions without citation

Protein kinase C, an elusive therapeutic target?
Mochly-Rosen et al. (2012)
34 more