› 3pg0

X-ray diffraction
1.62Å resolution

Crystal structure of designed 3-fold symmetric protein, ThreeFoil

Released:
DOI: 10.2210/pdb3pg0/pdb
PDB entry 3pg0 coloured by chain, front view.
PDB entry 3pg0 coloured by chain, side view.
PDB entry 3pg0 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Modular evolution and the origins of symmetry: reconstruction of a three-fold symmetric globular protein.
Broom A, Doxey AC, Lobsanov YD, Berthin LG, Rose DR, Howell PL, McConkey BJ, Meiering EM
Structure 20 161-71 (2012)
PMID: 22178248

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163884 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ThreeFoil Chain: A
Molecule details ›
Chain: A
Length: 165 amino acids
Theoretical weight: 18.06 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

3 bound ligands:
Ligand BTB 3 x BTB
Ligand GOL 2 x GOL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P43212
Unit cell:
a: 45.012Å b: 45.012Å c: 113.439Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.166 0.184
Expression system: Escherichia coli BL21(DE3)

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Citations

12 review citations

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Negahdaripour et al. (2017)
11 more

10 mentions without citation

Mushroom lectins: specificity, structure and bioactivity relevant to human disease.
Hassan et al. (2015)
9 more