› 3pht

X-ray diffraction
2.04Å resolution

Crystal structure of H74A mutant of Helicobacter Pylori NikR

Released:
DOI: 10.2210/pdb3pht/pdb
PDB entry 3pht coloured by chain, front view.
PDB entry 3pht coloured by chain, side view.
PDB entry 3pht coloured by chain, top view.
Source organism: Helicobacter pylori
Primary publication:
Ni(II) coordination to mixed sites modulates DNA binding of HpNikR via a long-range effect.
West AL, Evans SE, González JM, Carter LG, Tsuruta H, Pozharski E, Michel SL
Proc Natl Acad Sci U S A 109 5633-8 (2012)
PMID: 22451934

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127795 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative nickel-responsive regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 148 amino acids
Theoretical weight: 17.1 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli
UniProt:
  • Canonical: O25896 (Residues: 1-148; Coverage: 100%)
Gene name: HP_1338
Sequence domains:
Structure domains: ACT-like. Chain A, domain 2

Ligands and Environments

1 bound ligand:
Ligand NI 2 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P43212
Unit cell:
a: 73.174Å b: 73.174Å c: 114.441Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.23
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Metallochaperones and metalloregulation in bacteria.
Capdevila et al. (2017)
7 more

2 mentions without citation

Ni(II) coordination to mixed sites modulates DNA binding of HpNikR via a long-range effect.
West et al. (2012)
1 more