› 3pif

X-ray diffraction
2.92Å resolution

Crystal structure of the 5'->3' exoribonuclease Xrn1, E178Q mutant in Complex with Manganese

Released:
DOI: 10.2210/pdb3pif/pdb
PDB entry 3pif coloured by chain, front view.
PDB entry 3pif coloured by chain, side view.
PDB entry 3pif coloured by chain, top view.
Source organism: Kluyveromyces lactis
Primary publication:
Structural and biochemical studies of the 5'→3' exoribonuclease Xrn1.
Chang JH, Xiang S, Xiang K, Manley JL, Tong L
Nat Struct Mol Biol 18 270-6 (2011)
PMID: 21297639

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179438 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 116.267Å b: 132.581Å c: 144.066Å
α: 109.92° β: 105.81° γ: 104°
R-values:
R R work R free
0.249 0.248 0.284
Expression system: Escherichia coli

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Citations

18 review citations

RNA degradation in Saccharomyces cerevisae.
Parker R. (2012)
17 more

5 mentions without citation

Structural and biochemical studies of the 5'→3' exoribonuclease Xrn1.
Chang et al. (2011)
4 more