› 3pij

X-ray diffraction
1.8Å resolution

beta-fructofuranosidase from Bifidobacterium longum - complex with fructose

Released:
DOI: 10.2210/pdb3pij/pdb
PDB entry 3pij coloured by chain, front view.
PDB entry 3pij coloured by chain, side view.
PDB entry 3pij coloured by chain, top view.
Source organism: Bifidobacterium longum
Primary publication:
Crystal structures of the apo form of β-fructofuranosidase from Bifidobacterium longum and its complex with fructose.
Bujacz A, Jedrzejczak-Krzepkowska M, Bielecki S, Redzynia I, Bujacz G
FEBS J 278 1728-44 (2011)
PMID: 21418142

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106977 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
beta-fructofuranosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 526 amino acids
Theoretical weight: 59.17 KDa
Source organism: Bifidobacterium longum
Expression system: Escherichia coli
UniProt:
  • Canonical: A2TLS9 (Residues: 1-518; Coverage: 100%)
Gene names: B5U78_03730, CE169_09530, DWV59_08965, DWZ73_07715, DXC63_04880, GBC97_07305, GBI83_00975, GBI87_04425, GBK08_07415
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FRU 2 x FRU
Ligand CL 5 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P3121
Unit cell:
a: 86.765Å b: 86.765Å c: 223.868Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.154 0.153 0.208
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Specific and non-specific enzymes for furanosyl-containing conjugates: biosynthesis, metabolism, and chemo-enzymatic synthesis.
Chlubnova et al. (2012)
2 more

4 mentions without citation

Structure and evolution of the bifidobacterial carbohydrate metabolism proteins and enzymes.
Fushinobu et al. (2021)
3 more