› 3piu

X-ray diffraction
1.35Å resolution

High-resolution structure of native Malus domestica ACC synthase

Released:
DOI: 10.2210/pdb3piu/pdb
PDB entry 3piu coloured by chain, front view.
PDB entry 3piu coloured by chain, side view.
PDB entry 3piu coloured by chain, top view.
Source organism: Malus domestica
Primary publication:
Structural basis for reduced activity of 1-aminocyclopropane-1-carboxylate synthase affected by a mutation linked to andromonoecy.
Schärer MA, Eliot AC, Grütter MG, Capitani G
FEBS Lett 585 111-4 (2011)
PMID: 21075107

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine = 1-aminocyclopropane-1-carboxylate + S-methyl-5'-thioadenosine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153493 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-aminocyclopropane-1-carboxylate synthase Chain: A
Molecule details ›
Chain: A
Length: 435 amino acids
Theoretical weight: 49.32 KDa
Source organism: Malus domestica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37821 (Residues: 1-435; Coverage: 92%)
Gene name: ACS-1
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PLR 1 x PLR
No bound ligands
1 modified residue:
Ligand LLP 1 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM1A
Spacegroup: C2
Unit cell:
a: 103.71Å b: 61.14Å c: 76.83Å
α: 90° β: 123.12° γ: 90°
R-values:
R R work R free
0.149 0.149 0.171
Expression system: Escherichia coli BL21(DE3)

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Citations

5 citation in other articles

Molecular cloning and biochemical characterization of isoprene synthases from the tropical trees Ficus virgata, Ficus septica, and Casuarina equisetifolia.
Oku et al. (2015)
4 more

2 mentions without citation

Protein Phosphatases Type 2C Group A Interact with and Regulate the Stability of ACC Synthase 7 in Arabidopsis.
Marczak et al. (2020)
1 more