› 3pjs

X-ray diffraction
3.8Å resolution

Mechanism of Activation Gating in the Full-Length KcsA K+ Channel

Released:
DOI: 10.2210/pdb3pjs/pdb
PDB entry 3pjs coloured by chain, front view.
PDB entry 3pjs coloured by chain, side view.
PDB entry 3pjs coloured by chain, top view.
Source organisms:
Primary publication:
Mechanism of activation gating in the full-length KcsA K+ channel.
Uysal S, Cuello LG, Cortes DM, Koide S, Kossiakoff AA, Perozo E
Proc Natl Acad Sci U S A 108 11896-9 (2011)
PMID: 21730186

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209474 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
FAB light chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 215 amino acids
Theoretical weight: 23.56 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
FAB heavy chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 224 amino acids
Theoretical weight: 23.91 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
pH-gated potassium channel KcsA Chains: K, L, M, N
Molecule details ›
Chains: K, L, M, N
Length: 166 amino acids
Theoretical weight: 18.51 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 22-160; Coverage: 87%)
Gene names: kcsA, skc1
Sequence domains: Ion channel
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: I222
Unit cell:
a: 118.266Å b: 176.716Å c: 340.466Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.281 0.281 0.332
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Synthetic antibody technologies.
Adams et al. (2014)
5 more

13 mentions without citation

Mechanism of activation gating in the full-length KcsA K+ channel.
Uysal et al. (2011)
12 more