› 3pjv

X-ray diffraction
1.77Å resolution

Structure of Pseudomonas fluorescence LapD periplasmic domain

Released:
DOI: 10.2210/pdb3pjv/pdb
PDB entry 3pjv coloured by chain, front view.
PDB entry 3pjv coloured by chain, side view.
PDB entry 3pjv coloured by chain, top view.
Source organism: Pseudomonas fluorescens Pf0-1
Primary publication:
Structural basis for c-di-GMP-mediated inside-out signaling controlling periplasmic proteolysis.
Navarro MV, Newell PD, Krasteva PV, Chatterjee D, Madden DR, O'Toole GA, Sondermann H
PLoS Biol 9 e1000588 (2011)
PMID: 21304926

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174711 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diguanylate cyclase/phosphodiesterase Chains: D, F
Molecule details ›
Chains: D, F
Length: 130 amino acids
Theoretical weight: 14.26 KDa
Source organism: Pseudomonas fluorescens Pf0-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3KK31 (Residues: 22-151; Coverage: 20%)
Gene names: Pfl01_0131, lapD
Sequence domains: LapD/MoxY periplasmic domain
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 41.488Å b: 71.408Å c: 110.412Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.203 0.224
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.
Römling et al. (2013)
26 more

6 mentions without citation

Sensing the messenger: the diverse ways that bacteria signal through c-di-GMP.
Krasteva et al. (2012)
5 more