› 3pjz

X-ray diffraction
3.51Å resolution

Crystal Structure of the Potassium Transporter TrkH from Vibrio parahaemolyticus

Released:
DOI: 10.2210/pdb3pjz/pdb
PDB entry 3pjz coloured by chain, front view.
PDB entry 3pjz coloured by chain, side view.
PDB entry 3pjz coloured by chain, top view.
Source organism: Vibrio parahaemolyticus
Primary publication:
Crystal structure of a potassium ion transporter, TrkH.
Cao Y, Jin X, Huang H, Derebe MG, Levin EJ, Kabaleeswaran V, Pan Y, Punta M, Love J, Weng J, Quick M, Ye S, Kloss B, Bruni R, Martinez-Hackert E, Hendrickson WA, Rost B, Javitch JA, Rajashankar KR, Jiang Y, Zhou M
Nature 471 336-40 (2011)
PMID: 21317882

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183381 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trk system potassium uptake protein TrkH Chains: A, B
Molecule details ›
Chains: A, B
Length: 494 amino acids
Theoretical weight: 54.11 KDa
Source organism: Vibrio parahaemolyticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q87TN7 (Residues: 1-485; Coverage: 100%)
Gene names: VP0032, trkH
Sequence domains: Cation transport protein

Ligands and Environments

1 bound ligand:
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A, APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 91.489Å b: 97.406Å c: 195.653Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.249 0.299
Expression system: Escherichia coli BL21(DE3)

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Citations

24 review citations

The bacterial phosphoenolpyruvate:carbohydrate phosphotransferase system: regulation by protein phosphorylation and phosphorylation-dependent protein-protein interactions.
Deutscher et al. (2014)
23 more

12 mentions without citation

Plant High-Affinity Potassium (HKT) Transporters involved in salinity tolerance: structural insights to probe differences in ion selectivity.
Waters et al. (2013)
11 more