› 3pkz

X-ray diffraction
1.8Å resolution

Structural basis for catalytic activation of a serine recombinase

Released:
DOI: 10.2210/pdb3pkz/pdb
PDB entry 3pkz coloured by chain, front view.
PDB entry 3pkz coloured by chain, side view.
PDB entry 3pkz coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structural basis for catalytic activation of a serine recombinase.
Keenholtz RA, Rowland SJ, Boocock MR, Stark WM, Rice PA
Structure 19 799-809 (2011)
PMID: 21645851

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148969 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative transposon Tn552 DNA-invertase bin3 Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 124 amino acids
Theoretical weight: 14.38 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P20384 (Residues: 1-124; Coverage: 61%)
Gene name: bin3
Sequence domains: Resolvase, N terminal domain
Structure domains: Resolvase, N-terminal catalytic domain

Ligands and Environments

3 bound ligands:
Ligand SO4 20 x SO4
Ligand EDO 2 x EDO
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P65
Unit cell:
a: 128.208Å b: 128.208Å c: 182.015Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.207 0.246
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Mechanisms of DNA Transposition.
Hickman et al. (2015)
3 more

8 mentions without citation

Serine Resolvases.
Rice PA. (2015)
7 more