› 3pm1

X-ray diffraction
2.8Å resolution

Structure of QacR E90Q bound to Ethidium

Released:
DOI: 10.2210/pdb3pm1/pdb
PDB entry 3pm1 coloured by chain, front view.
PDB entry 3pm1 coloured by chain, side view.
PDB entry 3pm1 coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus Mu50
Primary publication:
A single acidic residue can guide binding site selection but does not govern QacR cationic-drug affinity.
Peters KM, Brooks BE, Schumacher MA, Skurray RA, Brennan RG, Brown MH
PLoS One 6 e15974 (2011)
PMID: 21264225

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140955 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator QacR Chains: A, B
Molecule details ›
Chains: A, B
Length: 194 amino acids
Theoretical weight: 22.94 KDa
Source organism: Staphylococcus aureus subsp. aureus Mu50
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: P0A0N3 (Residues: 1-188; Coverage: 100%)
Gene names: SAVP031, qacR
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 7 x SO4
Ligand ET 1 x ET
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P62
Unit cell:
a: 104.148Å b: 104.148Å c: 98.564Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.229 0.28
Expression system: Escherichia coli DH5[alpha]

Quick links

Citations

1 review citation

Revisiting ligand-induced conformational changes in proteins: essence, advancements, implications and future challenges.
Ahmad et al. (2013)
8 other articles

1 mention without citation

A single acidic residue can guide binding site selection but does not govern QacR cationic-drug affinity.
Peters et al. (2011)