› 3poq

X-ray diffraction
1.9Å resolution

Crystal structure of E.coli OmpF porin in lipidic cubic phase: space group H32, small unit cell

Released:
DOI: 10.2210/pdb3poq/pdb
Model geometry
Fit model/data
PDB entry 3poq coloured by chain, front view.
PDB entry 3poq coloured by chain, side view.
PDB entry 3poq coloured by chain, top view.
Source organism: Escherichia coli BL21
Primary publication:
Structure of Escherichia coli OmpF porin from lipidic mesophase.
Efremov RG, Sazanov LA
J Struct Biol 178 311-8 (2012)
PMID: 22484237

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136399 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane porin F Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 37.11 KDa
Source organism: Escherichia coli BL21
UniProt:
  • Canonical: P02931 (Residues: 23-362; Coverage: 100%)
Gene names: JW0912, b0929, cmlB, coa, cry, ompF, tolF
Sequence domains: Gram-negative porin
Structure domains: Porin

Ligands and Environments

2 bound ligands:
Ligand OLC 23 x OLC
Ligand SCN 3 x SCN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06DA
Spacegroup: R32
Unit cell:
a: 77.31Å b: 77.31Å c: 330.36Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.227 0.28

Quick links

Citations

2 review citations

Emerging Diversity in Lipid-Protein Interactions.
Corradi et al. (2019)
1 more

5 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
4 more