› 3pp4

X-ray diffraction
1.6Å resolution

Epitope characterization and crystal structure of GA101 provide insights into the molecular basis for the type I / type II distinction of anti- CD20 antibodies

Released:
DOI: 10.2210/pdb3pp4/pdb
PDB entry 3pp4 coloured by chain, front view.
PDB entry 3pp4 coloured by chain, side view.
PDB entry 3pp4 coloured by chain, top view.
Source organisms:
Primary publication:
Epitope characterization and crystal structure of GA101 provide insights into the molecular basis for type I/II distinction of CD20 antibodies.
Niederfellner G, Lammens A, Mundigl O, Georges GJ, Schaefer W, Schwaiger M, Franke A, Wiechmann K, Jenewein S, Slootstra JW, Timmerman P, Brännström A, Lindstrom F, Mössner E, Umana P, Hopfner KP, Klein C
Blood 118 358-67 (2011)
PMID: 21444918

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210510 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
GA101 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 224 amino acids
Theoretical weight: 23.97 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
GA101 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 219 amino acids
Theoretical weight: 23.96 KDa
Source organism: Mus musculus
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
B-lymphocyte antigen CD20 Chain: P
Molecule details ›
Chain: P
Length: 25 amino acids
Theoretical weight: 2.85 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P11836 (Residues: 163-187; Coverage: 8%)
Gene names: CD20, MS4A1

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 84.05Å b: 85.9Å c: 72.87Å
α: 90° β: 116.34° γ: 90°
R-values:
R R work R free
0.139 0.137 0.187
Expression systems:
  • Cricetulus griseus
  • Not provided

Quick links

Citations

52 review citations

The regulation and function of CD20: an "enigma" of B-cell biology and targeted therapy.
Pavlasova et al. (2020)
51 more

7 mentions without citation

Receptor-Targeted Nipah Virus Glycoproteins Improve Cell-Type Selective Gene Delivery and Reveal a Preference for Membrane-Proximal Cell Attachment.
Bender et al. (2016)
6 more