› 3ppb

X-ray diffraction
2.1Å resolution

Crystal structure of a putative tetR family transcription regulator (Shew_3104) from SHEWANELLA SP. PV-4 at 2.10 A resolution

Released:
DOI: 10.2210/pdb3ppb/pdb
PDB entry 3ppb coloured by chain, front view.
PDB entry 3ppb coloured by chain, side view.
PDB entry 3ppb coloured by chain, top view.
Source organism: Shewanella loihica PV-4
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107216 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 195 amino acids
Theoretical weight: 22.09 KDa
Source organism: Shewanella loihica PV-4
Expression system: Escherichia coli
UniProt:
  • Canonical: A3QHM2 (Residues: 1-194; Coverage: 100%)
Gene name: Shew_3104
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

4 bound ligands:
Ligand CL 2 x CL
Ligand EDO 10 x EDO
Ligand PEG 3 x PEG
Ligand PG4 3 x PG4
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 57.762Å b: 60.042Å c: 107.734Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.209
Expression system: Escherichia coli

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