› 3pqf

X-ray diffraction
2.49Å resolution

Crystal structure of L-lactate dehydrogenase from Bacillus subtilis mutation H171C complexed with NAD+

Released:
DOI: 10.2210/pdb3pqf/pdb
PDB entry 3pqf coloured by chain, front view.
PDB entry 3pqf coloured by chain, side view.
PDB entry 3pqf coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Zhang Y, Garavito RM

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146720 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 326 amino acids
Theoretical weight: 35.64 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13714 (Residues: 1-320; Coverage: 100%)
Gene names: BSU03050, lctE, ldh
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P31
Unit cell:
a: 133.431Å b: 133.431Å c: 99.088Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.189 0.235
Expression system: Escherichia coli BL21(DE3)

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