› 3pqr

X-ray diffraction
2.85Å resolution

Crystal structure of Metarhodopsin II in complex with a C-terminal peptide derived from the Galpha subunit of transducin

Released:
DOI: 10.2210/pdb3pqr/pdb
PDB entry 3pqr coloured by chain, front view.
PDB entry 3pqr coloured by chain, side view.
PDB entry 3pqr coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal structure of metarhodopsin II.
Choe HW, Kim YJ, Park JH, Morizumi T, Pai EF, Krauss N, Hofmann KP, Scheerer P, Ernst OP
Nature 471 651-5 (2011)
PMID: 21389988

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-136066 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Rhodopsin Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 39.03 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P02699 (Residues: 1-348; Coverage: 100%)
Gene name: RHO
Sequence domains:
Structure domains: Rhodopsin 7-helix transmembrane proteins
Guanine nucleotide-binding protein G(t) subunit alpha-1 Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.26 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P04695 (Residues: 340-350; Coverage: 3%)
Gene name: GNAT1

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
6 bound ligands:
Ligand RET 1 x RET
Ligand NAG 1 x NAG
Ligand BOG 2 x BOG
Ligand PLM 1 x PLM
Ligand SO4 1 x SO4
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: R32
Unit cell:
a: 241.55Å b: 241.55Å c: 109.87Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.217 0.25
Expression system: Not provided

Quick links

Citations

96 review citations

Molecular signatures of G-protein-coupled receptors.
Venkatakrishnan et al. (2013)
95 more

98 mentions without citation

Structure-function of the G protein-coupled receptor superfamily.
Katritch et al. (2013)
97 more