› 3pt9

X-ray diffraction
2.5Å resolution

Crystal structure of mouse DNMT1(731-1602) in the free state

Released:
DOI: 10.2210/pdb3pt9/pdb
PDB entry 3pt9 coloured by chain, front view.
PDB entry 3pt9 coloured by chain, side view.
PDB entry 3pt9 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of DNMT1-DNA complex reveals a role for autoinhibition in maintenance DNA methylation.
Song J, Rechkoblit O, Bestor TH, Patel DJ
Science 331 1036-40 (2011)
PMID: 21163962

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146802 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA (cytosine-5)-methyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 873 amino acids
Theoretical weight: 98.98 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13864 (Residues: 731-1602; Coverage: 54%)
Gene names: Dnmt, Dnmt1, Met1, Uim
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 75.567Å b: 79.685Å c: 162.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.256
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

93 review citations

DNA methylation: roles in mammalian development.
Smith et al. (2013)
92 more

7 mentions without citation

Modulation of epigenetic targets for anticancer therapy: clinicopathological relevance, structural data and drug discovery perspectives.
Andreoli et al. (2013)
6 more