› 3pu0

X-ray diffraction
3.09Å resolution

Crystal Structure of a vesicular stomatitis virus nucleocapsid-polyC complex

Released:
DOI: 10.2210/pdb3pu0/pdb
Model geometry
Fit model/data
PDB entry 3pu0 coloured by chain, front view.
PDB entry 3pu0 coloured by chain, side view.
PDB entry 3pu0 coloured by chain, top view.
Source organisms:
Primary publication:
Access to RNA encapsidated in the nucleocapsid of vesicular stomatitis virus.
Green TJ, Rowse M, Tsao J, Kang J, Ge P, Zhou ZH, Luo M
J Virol 85 2714-22 (2011)
PMID: 21177817

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137103 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Nucleoprotein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 421 amino acids
Theoretical weight: 47.33 KDa
Source organism: Vesicular stomatitis Indiana virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03521 (Residues: 2-422; Coverage: 100%)
Gene name: N
Sequence domains: Rhabdovirus nucleocapsid protein
Structure domains:
RNA (45-MER) Chain: R
Molecule details ›
Chain: R
Length: 45 nucleotides
Theoretical weight: 13.69 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand IUM 20 x IUM
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21212
Unit cell:
a: 164.652Å b: 235.554Å c: 75.857Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.25 0.29
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

9 review citations

Interplay between innate immunity and negative-strand RNA viruses: towards a rational model.
Gerlier et al. (2011)
8 more