› 3px8

X-ray diffraction
1.29Å resolution

RTA in complex with 7-carboxy-pterin

Released:
DOI: 10.2210/pdb3px8/pdb
PDB entry 3px8 coloured by chain, front view.
PDB entry 3px8 coloured by chain, side view.
PDB entry 3px8 coloured by chain, top view.
Source organism: Ricinus communis
Primary publication:
7-Substituted pterins provide a new direction for ricin A chain inhibitors.
Pruet JM, Jasheway KR, Manzano LA, Bai Y, Anslyn EV, Robertus JD
Eur J Med Chem 46 3608-15 (2011)
PMID: 21641093

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ricin A chain Chain: X
Molecule details ›
Chain: X
Length: 258 amino acids
Theoretical weight: 28.94 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02879 (Residues: 39-296; Coverage: 48%)
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand JP2 1 x JP2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21
Unit cell:
a: 42.662Å b: 67.567Å c: 49.41Å
α: 90° β: 112.78° γ: 90°
R-values:
R R work R free
0.208 0.206 0.233
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Ricinus communis intoxications in human and veterinary medicine-a summary of real cases.
Worbs et al. (2011)
2 more

3 mentions without citation

7-Substituted pterins provide a new direction for ricin A chain inhibitors.
Pruet et al. (2011)
2 more