› 3pxe

X-ray diffraction
2.85Å resolution

Impact of BRCA1 BRCT domain missense substitutions on phospho-peptide recognition: E1836K

Released:
DOI: 10.2210/pdb3pxe/pdb
PDB entry 3pxe coloured by chain, front view.
PDB entry 3pxe coloured by chain, side view.
PDB entry 3pxe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Impact of BRCA1 BRCT domain missense substitutions on phosphopeptide recognition.
Coquelle N, Green R, Glover JN
Biochemistry 50 4579-89 (2011)
PMID: 21473589

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153634 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Breast cancer type 1 susceptibility protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 214 amino acids
Theoretical weight: 24.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P38398 (Residues: 1646-1859; Coverage: 12%)
Gene names: BRCA1, RNF53
Sequence domains: BRCA1 C Terminus (BRCT) domain
Structure domains: BRCT domain
phospho peptide Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 10 amino acids
Theoretical weight: 1.21 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 4 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2221
Unit cell:
a: 115.82Å b: 131.05Å c: 180.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.22 0.266
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

4 review citations

Recent discoveries in the molecular pathogenesis of the inherited bone marrow failure syndrome Fanconi anemia.
Mamrak et al. (2017)
3 more

4 mentions without citation

Fanconi anemia pathway as a prospective target for cancer intervention.
Liu et al. (2020)
3 more