› 3q0m

X-ray diffraction
2.7Å resolution

Crystal structure of the PUMILIO-homology domain from Human PUMILIO1 in complex with p38alpha NREb

Released:
DOI: 10.2210/pdb3q0m/pdb
PDB entry 3q0m coloured by chain, front view.
PDB entry 3q0m coloured by chain, side view.
PDB entry 3q0m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Alternate modes of cognate RNA recognition by human PUMILIO proteins.
Lu G, Hall TM
Structure 19 361-7 (2011)
PMID: 21397187

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172069 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Pumilio homolog 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 349 amino acids
Theoretical weight: 40.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14671 (Residues: 828-1176; Coverage: 29%)
Gene names: KIAA0099, PUM1, PUMH1
Sequence domains: Pumilio-family RNA binding repeat
Structure domains: Leucine-rich Repeat Variant
5'-R(UP*GP*UP*AP*GP*AP*UP*A)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 8 nucleotides
Theoretical weight: 2.55 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 35.857Å b: 59.654Å c: 333.584Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.212 0.298
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

9 review citations

Engineering RNA-binding proteins for biology.
Chen et al. (2013)
8 more

3 mentions without citation

Role of PUM RNA-Binding Proteins in Cancer.
Smialek et al. (2021)
2 more