› 3q2j

X-ray diffraction
2.15Å resolution

Crystal Structure of 3',5"-Aminoglycoside Phosphotransferase Type IIIa Protein Kinase Inhibitor CKI-7 Complex

Released:
DOI: 10.2210/pdb3q2j/pdb
PDB entry 3q2j coloured by chain, front view.
PDB entry 3q2j coloured by chain, side view.
PDB entry 3q2j coloured by chain, top view.
Source organism: Enterococcus faecalis
Primary publication:
Crystal structures of two aminoglycoside kinases bound with a eukaryotic protein kinase inhibitor.
Fong DH, Xiong B, Hwang J, Berghuis AM
PLoS One 6 e19589 (2011)
PMID: 21573013

Function and Biology Details

Reaction catalysed: 
ATP + kanamycin = ADP + kanamycin 3'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141234 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside 3'-phosphotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 31.01 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A3Y5 (Residues: 1-264; Coverage: 100%)
Gene name: aphA
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand CKI 2 x CKI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 49.837Å b: 91.852Å c: 131.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.189 0.235
Expression system: Escherichia coli BL21(DE3)

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Citations

5 review citations

Antibiotic Resistance Mechanisms in Bacteria: Relationships Between Resistance Determinants of Antibiotic Producers, Environmental Bacteria, and Clinical Pathogens.
Peterson et al. (2018)
4 more

3 mentions without citation

Strategies to overcome the action of aminoglycoside-modifying enzymes for treating resistant bacterial infections.
Labby et al. (2013)
2 more